Anti Tetranectin (human)
mouse monoclonal antibody
Cat.No. HYB 130-13
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Tetranectin (TN) is a serum and tissue protein, a C-type lectin, which binds to Ca++. It is a homotrimer of monomers each with a mass of 20 kDa, plasma or serum concentrations of TN are found to be approximately 10 mg/l (1,2,4). In vitro, TN can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine (3). TN is best known as a prognostic marker in ovarian cancer.
Tetranectin purified from human citrate plasma (3) and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli.
HYB 130-13 is specific for amino acids 17-181 of human tetranectin monomer
Epitopespecificity is shared with HYB 130-11, as determined by inhibition ELISA (4).
HYB 130-13 reacts strongly with tetranectin. Strong reaction is seen in sandwich ELISA in combination with a polyclonal antibody against tetranectin (eg. DAKO A0371). In western blotting HYB 130-13 reacts strongly with TN monomer and slightly with TN trimer. In fresh frozen tissues of ovarian cancer, HYB 130-13 shows no staining for TN and no staining of paraffin-embeded, microwave treated tissues. Especially good as both capure and detecting antibody combined with a polyclonal antibody (A371) in sandwich ELISA (4).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | 4, 5 |
| Immunoblotting | Yes | 4 | |
| Immunohistochemistry | No |
1. Holtet TL, Graversen JH, Clemmensen I, Thogersen HC, Etzerodt M (1997) Tetranectin, a trimeric plasminogen binding C-type lectin. Protein Sci 6:1-5.
2. Nielsen BB, Kastrup JS, Rasmussen H, Holtet TL, Graversen JH, Etzerodt M, Thogersen HC, Larsen IK (1997) Crystal structure of tetranectin, a trimeric plasminogen-binding protein with an alpha-helical coiled coil. FEBS Lett. 412:388-96.
3. Clemmensen I, Petersen LC, Kluft C (1986) Purification and characterization of a novel, oligomeric, plasminogen kringle 4 binding protein from human plasma: Tetranectin. Eur J Biochem 156:327-33.
4. Hogdall CK, Christiansen M, Christensen L, Yazova AK, Koch C, Clemmensen I, Norgaard-Pedersen B (1997) Monoclonal antibodies against human tetranectin, epitope characterization and use in immunohistochemistry. Clin Chim Acta 258:159-177.
5. Hogdall CK (1998) Human tetranectin: methodological and clinical studies. APMIS Suppl 86:1-31.
6. Thougaard AV, Hogdall CK, Kjaer SK, Blaakaer J, Jaliashvili I, Christiansen M (1998) Determination of serum tetranectin: technical and clinical evaluation of three sandwich immunoassays. Clin Chim Acta 276:19-34.
7. Thougaard AV, Jaliashvili I, Christiansen M (2001) Tetranectin-like protein in vertebrate serum: a comparative immunochemical analysis. Comp Biochem Physiol B Biochem Mol Biol 128:625-634.
8. Westergaard UB, Andersen MH, Heegaard CW, Fedosov SN, Petersen TE (2003) Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator. Eur J Biochem 270:1850-1854.
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April 23 2008
